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Anti -SF3B14 Rabbit pAb

貨號:GB111446

品牌:Servicebio

價格: ¥ 650

規格: 100 μl

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Product Information

 

Description SF3B14 rabbit polyclonal
Protein full name Splicing factor 3B subunit 6
Synonyms Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, Sf3b6, Sf3b14
Immunogen KLH conjugated Synthetic peptide
corresponding to Mouse SF3B14
Isotype IgG
Purity Affinity purification
Subcellular location Nucleus
Predicted MW. 14 kDa
Observed MW. 14 kDa
Uniprot ID Q9Y3B4, P59708

 


 

Applications

 

Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 testis, colon
IHC Human, Mouse, Rat 1: 500-1: 1000 colon cancer, colon, breast cancer, testis, brain
IF Human, Mouse, Rat 1: 500-1: 1000 brain, testis

 


 

Background

 

This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.

 


 

Images

 

Western blot analysis of SF3B14 (GB111446)
at dilution of 1: 1000
Lane 1: Hela cell lysate
Lane 2: MCF7 cell lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Mouse colon tissue lysate
Lane 5: Rat testis tissue lysate

Immunohistochemistry analysis of paraffin-embedded human colon cancer using SF3B14 (GB111446) at dilution of 1: 500
Immunohistochemistry analysis of paraffin-embedded mouse colon using
SF3B14 (GB111446) at dilution of 1: 500
Immunohistochemistry analysis of paraffin-embedded rat colon using
SF3B14 (GB111446) at dilution of 1: 500
Immunohistochemistry analysis of paraffin-embedded human breast cancer using SF3B14 (GB111446) at dilution of 1: 500
Immunohistochemistry analysis of paraffin-embedded mouse testis using
SF3B14 (GB111446) at dilution of 1: 500
Immunohistochemistry analysis of paraffin-embedded mouse brain using
SF3B14 (GB111446) at dilution of 1: 500
Immunohistochemistry analysis of paraffin-embedded rat brain using
SF3B14 (GB111446) at dilution of 1: 500
Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using SF3B14 (GB111446) at dilution of 1: 500
Immunofluorescent analysis of paraformaldehyde-fixed rat testis using
SF3B14 (GB111446) at dilution of 1: 500

 


 

Storage

 

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

 


 

NOTE: This product is intended for research only.

 

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